Antioxidant, Cytotoxicity Studies and Isolation of Trans-Anonene and Lupeol  from leaves and stem bark extracts of Croton membranaceus

Authors

M. S. Isyaka Department of Chemical Sciences, Faculty of Science and Computing, North-Eastern University, Gombe State Author
S. Jibril Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, Gombe, Nigeria Author
J. O. Igoli Department of Chemistry, College of Science, Joseph Sarwuan Tarka University, Makurdi, Benue State, Nigeria Author
Y. Rufai Chemistry Department, Federal College of Education, Okene, Kogi State, Nigeria Author
B. M. Mofio Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja Author
A. A. Sadam Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja Author
B. B. Nyakuma Department of Chemical Sciences, Faculty of Science and Computing, North-Eastern University, Gombe State Author
B. A. Adedoyin Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja Author
A., Umar Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja Author
T., Adamu Department of Chemistry, Faculty of Science, University of Abuja, Gwagwalada, FCT, Abuja Author
Z. Abdu Department of Chemical Sciences, Faculty of Science, Federal University of Kashere, Gombe, Nigeria Author

Antioxidant activity, Cytotoxicity, C. membranaceus, Trans-Anonene, Lupeol

The antioxidant activity of the dichloromethane (CH2Cl2) and methanol (MeOH) extracts of the

leaves and stem bark of Croton membranaceus was carried out using a 2,2-Diphenyl-1-picryl�

hydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and metal

chelation assay along with cytotoxicity analysis using the Brine shrimp lethality assay (BSLA).

The silica gel-based chromatographic separation of the CH2Cl2 and MeOH extracts successfully

isolated trans-annonene and lupeol. The structures of the isolated compounds were subsequently

identified using Fourier-transform infrared (FTIR) spectroscopy and nuclear magnetic resonance

(NMR) based on 1D and 2D data compared with the literature. The CH2Cl2 and MeOH extracts

exhibited antioxidant activity in a concentration-dependent manner but were highly cytotoxic. In

conclusion, the study showed that the leaves and stem bark of C. membranaceous are potentially

viable sources of bioactive compounds with potential health and medicinal benefits.