Production of Biobutanol by Clostridium Species using Liquid Pineapple Waste

Abstract

Biobutanol production by Clostridium species UPM-A1 strain was conducted with liquid pineapple waste as carbon source. Design Expert® Software was employed for generating the experimental design which consisted of three significant parameters, including temperature, yeast extract, and pH. The design consisted of twenty (20) different experiments including six (6) replicates at central points. The experiments were conducted in accordance with the conditions provided by the software, during which the highest biobutanol production was attained at run three (3) consisting, temperature 42.50 0C, yeast extract 2.14 g/L, and pH 5.5, with a total biobutanol concentration value of 0.441 g/L. After all, a model was generated to see the effects of the variables on the biobutanol produced. Furthermore, the software suggested optimum values for each parameter as temperature 49.35 0C, yeast extract 1.80 g/L, and pH 4 with a biobutanol concentration of 0.405 g/L. A laboratory experiment was conducted to test the predicted variables and eventually 0.417 g/L of biobutanol concentrations was achieved, which is slightly higher than the predicted. The research, eventually, provided a bottom-up method towards reducing the number of lignocellulose wastes from environments through the production of valuable products.